Fitness Measurements of Evolved Esherichia coli
نویسندگان
چکیده
منابع مشابه
Study the Expression of ompf Gene in Esherichia coli Mutants
The outer membrane porin proteins are the major factors in controlling the permeability of cell membrane. OmpF is an example of porin proteins in Esherichia coli. In normal growth condition a large amount of this protein is synthesised, but under stress condition, such as the presence of antibiotics in environment its expression is decreased inhibiting the entrance of antibiotics into cell. The...
متن کاملImpact of individual mutations on increased fitness in adaptively evolved strains of Escherichia coli.
We measured the relative fitness among a set of experimentally evolved Escherichia coli strains differing by a small number of adaptive mutations by directly measuring allelic frequencies in head-to-head competitions using a mass spectrometry-based method. We compared the relative effects of mutations to the same or similar genes acquired in multiple strains when expressed in allele replacement...
متن کاملMeasurements of fitness and competition in commensal Escherichia coli and E. coli O157:H7 strains.
Although the main reservoirs for pathogenic Escherichia coli O157:H7 are cattle and the cattle environment, factors that affect its tenure in the bovine host and its survival outside humans and cattle have not been well studied. It is also not understood what physiological properties, if any, distinguish these pathogens from commensal counterparts that live as normal members of the human and bo...
متن کاملFitness, environment and input: Evolved robotic shepherding
A simple shepherding task is used as a framework in which to investigate the role played by fitness functions, environmental design and inputs to a neural network in the evolution of robotic behavior. While a cursory approach to this experimental problem might suggest that the only factor relevant to success is the function used to gauge a robotic brain’s fitness, experimental results indicate ...
متن کاملStudy the Expression of marA Gene in Ciprofloxacin and Tetracycline Resistant Mutants of Esherichia coli
MarA activates two membrane dependent mechanisms of resistance to different antibiotics, such as ciprofloxacin and tetracycline, including promotion of outflux and inhibition of influx of antibiotics. Thus, MarA causes multiple antibiotic resistance phenotype. The activation of these mechanisms needs overexpression of marA. This could happen through mutation in marR. Thus, the aim of this study...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: BIO-PROTOCOL
سال: 2014
ISSN: 2331-8325
DOI: 10.21769/bioprotoc.1228